sliding microtome

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Sliding Microtome INSTRUCTION MANUAL

The Thermo Scientific Sliding Microtome Microm HM 450 is a highly efficient sliding microtome for paraffin sectioning in routine Only skilled or specially trained personnel must operate the microtome i e clamping the specimen trimming sectioning and taking off the sections from the instrument

What Is microtome?

A microtome is a mechanical device used to slice biological specimens into extremely thin segments for microscopic examination.Micro = Small, and Tome = Sliced. Tissue blocks embedded in paraffin wax are most commonly sectioned for microtomy, but the tissue can also be presented in other forms.A microtome into which a cutting instrument is clamped is the fundamental microtomy implement. Cummings (1770), a manufacturing company, created wooden manual sectioning instruments that were used

Parts of A Microtome

There are several key parts of a microtome, including: 1. Base: The bottom part of the microtome that provides stability and support. 2. Sample holder: A platform or clamp that holds the material being sliced in place. 3. Cutting mechanism: A system that moves a blade or knife across the material being sliced to create thin sections. 4. Feed mechan

Types of Microtomes

It is the primary instrument used to cut narrow sections of tissue embedded in paraffin blocks. The various varieties of microtomes utilized in a conventional histology laboratory are: Microtomes can be classified as: 1. Manual microtomes 2. Semi- automatic microtomes 3. Automatic microtomes microbiologynote.com

A. Manual Microtomes

A manual microtome is a type of microtome that is operated by hand, rather than using an automated or electric system. Manual microtomes are often used in laboratories and research settings where precision and control are critical. Some of the key features and benefits of manual microtomes include: 1. Precision: Manual microtomes allow for very pre

B. Automatic Microtomes

An automatic microtome is a type of microtome that uses an electric or automated system to control the slicing process. Automatic microtomes are often used in high-volume or industrial settings, where speed and efficiency are critical. Some of the key features and benefits of automatic microtomes include: 1. Speed: Automatic microtomes can slice th

Microtome Knife

The microtome knife plays a crucial role in creating high-quality sections. The initial attempt at creating a suitable cutting surface was a razor blade that had been sharpened; however, these quic

Types of Microtome Knife Based on Material of The Knife

1. Steel Knives 1. High-quality carbon or tool-grade steel is used in the production of steel microtome knives, and the blades are heat treated to strengthen the edge. 2. The steel must be devoid of contaminants and corrosion inhibitors, and it must also resist rust. Completely hardened blades are the best kind. 3. When the hardened area is removed during sharpening, those with merely a surface hardening lose their cutting edge rapidly. 2. Non-Corrosive Knives For Cryostats 1. Made from pure, heat-treated stainless steel, these are extremely durable. Chrome levels range from 12 to 15 per cent. 3. Disposable Blades 1. You might think of these as improved, beefed-up razor blades. When placed in a specialized holder, these blades reliably cut clean pieces every time. 2. In many cases, these have completely supplanted traditional microtome knives. Stainless steel is used in the production of all disposable blades. 3. Platinum or chromium coatings can be applied to the cutting edge of disposable blades to make them stronger and last longer. Because of their low cutting resistance and low friction, Teflon-co

Types of Microtome Knife Based on Shape of The Knife Edge

1. PROFILE – A: Strongly plano concave/biconcave 1. The plano concave knife has one straight surface and one recessed ground surface. The biconcave blade of the knife features two hollow-ground surfaces. 2. Both blades are exceptionally sharp and are used to cut soft, celloidin-imbedded materials and foam compounds. 3. These blades are unsuitable for relatively hard materials, which cause the edge to vibrate and result in the chattering phenomenon. When cutting sections, the knife should always be at an angle to the object for optimal results. 2. PROFILE – B: Plano concave 1. This knife resembles a profile-A knife, but its rear is thicker. It is used to cut portions from materials that are too difficult to cut with a profile-knife, but it can also be used for paraffin-encased softer materials. 2. This knife should be held at an angle to the material being cut. There are plano-concave knives with differing degrees of concavity. 3. PROFILE – C: Wedge Shaped 1. The knife with a wedge shape is more rigid than blades with profile A or B and can therefore be used to cut harder materials. 2. Due to the extra-thick nature of the tip wedge, this form of knife cannot be ground as sharply as knives with profile A or B. 3. It is typically used for cutting sections from embedded paraffin wax, frozen sections, cryostat sections, and small embedded synthetic resin material. This design of knife has a cutting plane that is perpendicular to the object. microbiologynote.com

Angles of Knife

The various angles formed by the knife include 1. Rake angle:It is the angle between the upper chamfer of the knife and the line drawn perpendicularly from the surface of the block. Increasing the slope angle facilitates section cutting. 2. Angle of clearance: The angle between the knife’s lower bevel and the surface of the block. Typically, it is

Steps of Tissue Sectioning

Trimming the tissue: Tissue trimming is required to expose the tissue component within the paraffin wax for subsequent cutting. The block is secured in the microtome’s chuck, and the paraffin is re

Postbac Life: Lindsey Jay Demonstrates the Microtome

PDF	Thermo Scientific Sliding Microtome Microm HM 430 Instruction The sliding microtome HM 430 from Thermo Scientific is a highly efficient instrument for sectioning paraffin in routine and research. The main applications are

PDF	Thermo Scientific Sliding Microtome Microm HM 450 INSTRUCTION 28 окт. 2002 г. The sliding microtome HM 450 from Thermo Scientific is a highly efficient instrument with specimen retraction during return travel. It can be ...

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PDF	Thermo Scientific Sliding Microtome Microm HM 450 INSTRUCTION 28 oct 2002 Thermo Scientific Sliding Microtome Microm HM 450. Microm International GmbH part of Thermo Fisher Scientific. Otto-Hahn-Str. 1A.

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PDF	Thermo Scientific Sliding Microtome Microm HM 430 Instruction Thermo Scientific Sliding Microtome Microm HM 430. Microm International GmbH part of Thermo Fisher Scientific. Otto-Hahn-Str. 1A. 69190 Walldorf / Germany.

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PDF	Sliding Microtome PDF Sliding Microtome is a standard sectioning instrument especially designed for. Research Laboratories for cutting sections of paraffin as well as celloiden

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PDF	Sliding Microtome Microm HM 430 - 387756 Thermo Scientific Sliding Microtome Microm HM 430 Microm International GmbH part of Thermo Fisher Scientific Otto-Hahn-Str 1A 69190 Walldorf / Germany

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