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PDF 6 OUCHTERLONY DOUBLE IMMUNODIFFUSION TEST

OUCHTERLONY DOUBLE IMMUNODIFFUSION TEST Aim: To study the reaction pattern of an antigen with a set of antibodies by Ouchterlony Double Diffusion method Introduction: Immunodiffusion in gels encompasses a variety of techniques which are useful for the analysis of antigens and antibodies Gel immunodiffusion can be classified into two groups:

  • What is the Ouchterlony Test for bronchopulmonary aspergillosis?

    Currently, the gel diffusion (Ouchterlony) test is considered a classic method for identifying IgG precipitating antibodies (precipitins) to those antigens associated with hypersensitivity pneumonitis and allergic bronchopulmonary aspergillosis. In 1972, precipitins were detected to mold hay in the serum of Farmer’s lung patients (Pepys 1972 ).

  • What is an Ouchterlony double immunodiffusion plate?

    Picture of an Ouchterlony double immunodiffusion plate, after immunodiffusion has taken place. In this, titre value of an antigen is quantified. The central well has an antibody, and the surrounding wells have decreasing concentration of the corresponding antigen. Ouchterlony patterns showing no identity between upper spots

  • What is Ouchterlony double diffusion method?

    To study the reaction pattern of an antigen with a set of antibodies by Ouchterlony Double Diffusion method. Immunodiffusion in gels encompasses a variety of techniques, which are useful for the analysis of antigens and antibodies. Gel immunodiffusion can be classified into two groups:

Ouchterlony Assay

Ouchterlony Assay

Immunité adaptative 3 : Test dOuchterlony

Immunité adaptative 3 : Test dOuchterlony

The Ouchterlony Test

The Ouchterlony Test

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